This paper studies the fault detection and isolation problem for uncertain flight control systems with actuator outage faults, which has not been well investigated in the literature. A state feedback controller and a detection weighting matrix are designed simultaneously, through which the closed-loop system is stabilized for both fault-free and actuator outage cases, and the actuator outage faults can be detected through the residual signal generated by recombining system states with the detection weighting matrix. Additionally, a set of isolation weighting matrices are designed to identify the locations of the actuators that are of outage. A VTOL aircraft model is given to illustrate the effectiveness of the proposed methods.