The two echelon vehicle route decision strategies and the performance in rescue resource distribution system are analyzed. Firstly, a min-max two echelon vehicle routing model is developed. Then, for the main warehouse delay time uncertainty, four decision strategies are proposed, which are simple strategy, optimistic collaboration strategy, pessimistic collaboration strategy and mixed collaboration strategy. A multi-star iteration local research algorithm and simulation program are developed. Finally, numerical studies are presented to compare the impact of decision strategies on the relief performance, and the results show that mixed collaboration strategy is the best strategy in four strategies.