Pulse signal peak plays a significant role on the analysis and diagnosis of human physiological parameters. This paper proposes an adaptive pulse signal peak location method to deal with peak location of the pulse signal. The power frequency noise and baseline drift are removed previously in the pulse signal. Then fast Fourier transform(FFT) combines heart rate from the volunteers to calculate the single period step of the pulse signal. In a single period step of the pulse signal, the pulse signal peak can be located only with the amplitude difference. Experimental results show that, the peak detecting recall and precision of the method are improved significantly. Moreover, when the value of tide wave is similar to that of pulse peak, the method can avoid locating multiple peaks in a single period and decrease the error rate of peak position, which embodies the high practicality.