This paper is concerned with the problem of the stochastic stability analysis and controller design of the time-delay Markovian jump system with disturbance and actuator saturation. The stochastic stability problem of the closed-loop system with disturbance estimation error is analyzed. By constructing the appropriate mode-dependent Lyapunov-Krasovskii functions and introducing the free-connection weighting matrices, the random stability criterions of the closed-loop system are given. By transforming it into a feasible problem with linear matrix inequalities, the gain matrices are acquired. And the estimation of maximized attractive domain is obtained by an iterative optimization algorithm. Finally, the simulation results show the effectiveness of the proposed method.